Extended Data Fig. 7: Transcription factor networks in mouse radial glia cell types.
From: Distinct radial glia subtypes regulate midbrain dopaminergic neuron development
a–c, Clustering of TFs expressed in mRgl1 (a), mRgl2 (b), or mRgl3 (c) based on Jaccard index of shared target genes. d, Percentage of significant FWY combinations obtained by randomly selecting TFs from MSigDB while keeping mRgl1-3 transcriptomes constant. e, mRgl3 transcriptional network. Node color represents the number of TF connections (node degree), and node size reflects connection strength (weighted degree). Core nodes (high weighted degrees) are outlined in black. Edge width and color intensity reflect TF pair interaction scores. f, Scatterplot comparing TF node degree (connectivity) and weighted node degree (interaction strength) in mRgl3 network. Node size and color intensity correspond to weighted degree. g,h, GO analysis of target genes regulated by core TFs in the mRgl3 (g) and mRgl1 (h) TF networks, using MSigDB gene set C2 canonical pathways v5.0 and two-sided Fisher’s exact test with Benjamini–Hochberg correction. i, Selected target genes regulated by core TFs in the mRgl1 TF network. Target genes identified based on ref. 74. j, UMAP adapted from ref. 4 showing cells in the developing human VM annotated by enriched midbrain floor plate (FOXA2+/LMX1A+/EN1+) or anterior floor plate (FOXA2+/LMX1A+/PITX2+) identity. FWY, Fast-Westfall-Young.
