Fig. 3: Spatial transcriptomic analysis of mouse brain aging using IRISeq.
From: Optics-free spatial genomics for mapping mammalian brain aging by IRISeq
a, Schematic of IRISeq profiling across various brain regions, including the frontal isocortex, dorsal cortex, hippocampus, thalamus, hypothalamus and additional associated regions. ‘Section 1’ and ‘section 2’ are for the frontal and middle parts of the cortex, and ‘section 3’ is near the dorsal part of the cortex. b, Regional gene expression heatmap. c, Regional gene expression UMAP. d,e, Image reconstruction of all receiver beads derived from bead–bead connections (d), with coloring corresponding to the annotated regions based on gene expression (e). Schematic in a created in BioRender; Cao, J. https://biorender.com/c6n61lz (2026). AMY, amygdala; CA1–CA3, hippocampal cornu ammonis regions 1–3; CP, caudate putamen; CRTX_RA, retrosplenial cortical area; CTX, cortex/cortical area; CTX_A, cortical associated area; DG, dentate gyrus; Glia_Vascular, glia- and endothelial-rich region; HAB_V3, habenula/third ventricle region; Hipp_CA, hippocampal CA1–CA3 region; Hipp_DG, hippocampal dentate gyrus; HYP, hypothalamus; NA, nucleus accumbens; OLF_STR, olfactory and striatal area; SI, substantia innominata; THcv, central ventral thalamus; THrn, thalamic reticular nucleus; VEN, ventricles; V3, third ventricle; WM, white matter. S1–S3 indicate tissue sections 1–3, respectively.
