Extended Data Fig. 3: PC values at exit and return to NREM subspace, and PC activity prior to spontaneous and laser-induced REM sleep.
From: Low-dimensional population dynamics in the brainstem gate REM sleep
(a) PC1 and PC2 values at exit and return to the NREM subspace. Two-way repeated-measures (RM) ANOVA with PC and condition (Exit vs Return) as within factors; interaction, P = 0.00033, F(1,5) = 75.60; condition, P = 4.65×10−5, F(1,5) = 171.28; two-sided paired t-tests with Bonferroni correction; PC1: Exit vs Return, P = 4.45e-03, T(5) = 5.76; PC2: P = 1.95×10−6, T(5) = 28.62. Bars, mean; error bars, 95% CIs; dots, individual mice; n = 6 mice. **P < 0.01, ***P < 0.001. (b) Time course of PC1 and PC2 (z-scored) relative to REM→Wake transitions (at t = 0 s). PC2 values significantly differed from 0 at t = 0 s; two-sided one-sample t-test; P = 5.41×10−17; T(44) = -13.28; n = 45 REM→Wake transitions from 6 mice. Shadings, s.e.m. (c) Time course of PC1 and PC2 (z-scored) relative to NREM→REM and NREM→Wake transitions (at t = 0 s). The red lines indicate the time point tsig from when on PC1 or PC2 significantly differed prior to NREM→REM and NREM→Wake transitions. Two-sided paired t-tests with Bonferroni correction; PC1: P < 0.000045, tsig = -20 s; PC2: P < 0.048, tsig = -100 s; n = 47 NREM→REM, n = 190 NREM→Wake transitions. Shadings, s.e.m. See Supplementary Table 1 for full statistical details.
