Supplementary Figure 7: Tankyrase1 depletion does not affect telR-loop levels.
From: TRF1 participates in chromosome end protection by averting TRF2-dependent telomeric R loops
(a) Western blot analysis of HeLa and U2OS cells transfected with an siRNA against Tankyrase 1 (siTNK1) or a control siRNA (siCt) using anti-TNK1 and Lamin A and C (LMNA and LMNC; loading control) antibodies. Cells were harvested 4 days after transfections. (b) Cells as in a were stained with DAPI and mitotic cells were counted and graphed as means ± SD (n = 3 independent experiments). Examples of DAPI-stained mitotic cells (white arrowheads) are shown on the right. Scale bar, 30 μm. (c) Telomeric dot-blot hybridization of S9.6 DRIPs of cells as in a. In, input; Bd, beads only control; IP, S9.6 immunoprecipitated material. S9.6 DRIP quantifications are shown on the right. Signals are graphed as the fraction of In DNA found in IPs after subtraction of Bd-associated signal and normalization over siCt-transfected cells. Values are means ± SD (n = 4 independent experiments).
