Supplementary Figure 1: Control strand-invasion assays.
From: TRF1 participates in chromosome end protection by averting TRF2-dependent telomeric R loops
(a) Strand invasion assays with 0.6 nM pcDNA6 empty vector or pcDNA6 containing a ~800 bp long telomeric array (p-Tel) in combination with increasing amounts of the indicated RNA and DNA oligonucleotides. (b) Invaded plasmids in experiments as in a were quantified and graphed as the fold increase compared to reactions performed with 2 nM oligonucleotides. Values are means ± SD (n = 3 independent experiments). (c) Reaction products from invasion assays with the indicated oligonucleotides and the p-Tel plasmid were incubated with RNaseH (RH) before electrophoresis. (d) Strand invasion assays with TERRA-like oligonucleotides, the p-Tel plasmid and the indicated recombinant proteins were incubated with RNaseH before electrophoresis. GST-TRF2 concentration was 40 nM, GST-TRF1 and GST-TRF1ΔA concentrations were 0, 10, 20, 40 and 80 nM. inv, invaded plasmid; ss, single stranded RNA or DNA oligonucleotides; *, wells.
