Supplementary Figure 6: An N-terminal SUZ12 fragment is essential for CGI binding and correct H3K27 methylation patterns.
From: Accurate H3K27 methylation can be established de novo by SUZ12-directed PRC2
a, Heatmaps of sequence-depth normalized ChIP-seq data of H3K27 methylation in genic regions. Vertical axis contains all RefSeq genes > 250bp and horizontal axis is centered on genes represented by relative gene length. TSS: Transcription start site. Arrow ends at end of genes. b, Heatmaps of sequence-depth normalized ChIP-seq data of H3K27 methylation within random genomic regions. Vertical axis contains 50,000 random 5000 bp regions clustered (k-means clustering, k = 10) according to methylation state in E14 cells. Horizontal axis is 25,000 bp wide centered on regions used for clustering. The clusters are ordered according to highest methylation state in untreated cells. c, Heatmaps of sequence-depth normalized H3K27me1 ChIP-seq data clustered with H3K9me3 and H3K36me3. Regions in H3K27me1 positive clusters (8 and 9, 6670 regions) have been submitted to new clustering with published H3K9me3 and H3K36me3 data55 to yield clusters B1 to B6. Horizontal axis is 100,000 bp wide centered on regions (5000 bp) used for clustering.
