Supplementary Figure 3: Cryo-EM reconstruction of Xenopus TRPV4cryst.
From: Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms
a, Flowchart of cryo-EM data processing. b, Fourier shell correlation plot for half-maps calculated in FREALIGN. The overall resolution is estimated to be 3.84 Å on the basis of the FSC = 0.143 cut-off criterion. c, Angular distribution plot for particles in the reconstruction. d, Sharpened cryo-EM density map colored according to local resolution using ResMap.
