Supplementary Figure 4: uORF translation remodels 5′-UTR structure.
From: Analyses of mRNA structure dynamics identify embryonic gene regulatory programs
a, Schematic view of the 5’-UTR RNA structure remodeling that occurs upon translation of upstream open reading frames (uORF). b, Cumulative distributions of in vivo (top) and in vitro (bottom) uORF accessibilities for highly (red, n = 196) and lowly (blue, n = 196) translated uORFs. P-values were determined using one-sided Mann-Whitney U-tests. c, Distributions of ribo-seq reads (top panel) and accessibility differences (in vivo – in vitro) (bottom panel) for the cdc25 5’-UTR, which contains two translated uORFs (highlighted in gray). Increased accessibility in vivo is shown in red, whereas decreased accessibility in vivo is shown in blue. d, Arc plot depicting the predicted cdc25 5’-UTR secondary structure, guided by either in vivo (yellow) or in vitro (green) DMS-seq accessibilities. Each arc represents a base pair interaction
