Supplementary Figure 5: Poly(A) tail length–dependent mRNA structure remodeling depends on translation.
From: Analyses of mRNA structure dynamics identify embryonic gene regulatory programs
a, Comparison of per-region accessibilities (CDS; top panels and 3’-UTR; bottom panels) and per-transcript poly(A) tail length, at 2 hpf (left panels) and 4 hpf (right panels) (CDS 2 hpf (n = 1,238) and 4 hpf (n = 1,138; 3’-UTR 2 hpf (n = 1,221) and 4 hpf (n = 1,014)). Spearman correlation coefficients (rho) and P-values are shown. b, c, Comparison of CDS accessibilities and per-transcript poly(A) tail lengths in PatA-treated (b, n = 1,671) and in vitro refolded samples (c, n = 1,663) from 2 hpf embryos. Spearman correlation coefficients (rho) and P-values are shown. d, Comparison of differentially structured (DS) windows (2 vs 4 hpf) found in 483 miR-430 targets (red) and 1,000 sets of 483 randomly chosen non miR-430 target mRNAs (gray)
