Supplementary Figure 4: Yeast two-hybrid analysis of the interaction between Msh2 and Utp18 and Bir1 and analysis of the levels of Pms1–4 × GFP foci in dpb3Δ fun30Δ single-mutant strains.
From: Identification of Exo1-Msh2 interaction motifs in DNA mismatch repair and new Msh2-binding partners
a, Prey vectors bearing either BIR1 or UTP18 were co-transformed with either empty bait vectors or bait vectors bearing wild-type MSH2, and interactions were evaluated as described in the legends to Fig. 1 and Supplementary Fig. 1. All experiments were independently repeated a minimum of four times. b, Pms1–4 × GFP foci were monitored in logarithmically growing asynchronous cultures by fluorescence microscopy, and the fraction of cells with one or more Pms1–4 × GFP foci was expressed as the fold change over wild type. The average value ( ± s.d.) from three independent experiments is presented.
