Supplementary Figure 5: Extension of αvβ3 does not change its intrinsic ligand affinity; integrin β leg sequence alignment.
From: Cryo-EM structure of the αvβ8 integrin reveals a mechanism for stabilizing integrin extension
a, Receptor binding of αvβ3 ectodomains (WT and αvc-cβ3) to vitronectin-N in basal (Ca2+) or activating (Mn2+) cation conditions (n = 3, ± s.e.m.). b, Receptor binding of αvβ8 ectodomains (WT and αvc-cβ8) to L-TGF-β (n = 3, ± s.e.m.). c, Sequence alignment for the leg of all β integrins pairing with αv. Yellow highlights conserved cysteines in the β legs, notable exceptions in the β8 leg are highlighted in blue.
