Supplementary Figure 1: Molecular architecture of Pol II(G) by cryo-EM and CX-MS analyses.
From: Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1
a, SDS-PAGE analysis (sliver staining) of purified Pol II(G). b, Observation of individual Pol II(G) under cryo-EM conditions with a K2 direct electron detector. c, 2D classification result from the full Pol II(G) data set. d, Computational sorting of cryo-EM particle images and map reconstructions. Three-dimensional classification helps to select the homogenous particles. Only one class was selected and further subjected to movie processing and 3D Auto-Refinement. e, Local resolution of Pol II(G) cryo-EM map. The Pol II(G) ~4-Å cryo-EM map is colored by local resolution and shown as a surface (top) and slice through (bottom). f, FSC plot and resolution estimation using the gold-standard 0.143 criterion for the cryo-EM map, as well as the FSC plot for the Pol II(G) model versus the Pol II(G) map. g, Angular distribution plot for the Pol ll(G) cryo-EM image set.
