Supplementary Figure 1: FLNaABD–F-actin complexes.
From: Structural basis of the filamin A actin-binding domain interaction with F-actin
a, High-frequency noise-substituted Fourier shell correlation calculations between independently refined half maps reveals the resolution of each map. The FSC curve between the final FLNaABD-E254K–F-actin map and refined model is shown in purple. b, A 10-Å-filtered cryo-EM density map of wild-type (WT) FLNaABD (left and middle) was rigid-body docked with crystal structures for actin (PDB 6C1D; different subunits in dark blue, cyan, and light blue) and FLNaCH1 (PDB 3HOP; green) (right). c, A 6.6-Å-filtered cryo-EM density map of the gain-of-function mutant FLNaABD-Q170P (left and middle) was docked with the refined actin and FLNaCH1 models (right) and colored as in b. d, A 7.4-Å-filtered cryo-EM density map of the gain-of-function mutant FLNaABD-E254K (left and middle) was docked with the refined actin and FLNaCH1 models (right) and colored as in b. Observed extra density for FLNaCH2 is shown in tan.
