Supplementary Figure 2: ABS-N mutations inhibit FLNaABD–F-actin binding.
From: Structural basis of the filamin A actin-binding domain interaction with F-actin
a, Micrographs of mouse NIH-3T3 fibroblasts transiently transfected with GFP or FLNaABD-GFP ± N-terminal truncation constructs after fixation, permeabilization, and staining with Alexa Fluor 568–phalloidin to visualize F-actin filaments. Cells were imaged in the green (left; GFP signal) and red (right; F-actin signal) channels. Scale bar, 20 µm. WT, wild type. b, Anti-GFP immunoblot on lysates from NIH-3T3 cells transiently transfected with GFP or FLNaABD-GFP ± N-terminal truncations. Vinculin was used as a loading control. c, Micrographs of fibroblasts transfected with GFP or FLNaABD-GFP constructs containing ABS-N mutations, prepared and imaged as in a. Scale bar, 20 µm. d, Anti-GFP immunoblot on lysates from NIH-3T3 cells transiently transfected with GFP or FLNaABD-GFP ± ABS-N mutations. Vinculin was used as a loading control.
