Supplementary Figure 3: Immunoblotting of ABS2ʹ and ABS2 mutant FLNaABDs.
From: Structural basis of the filamin A actin-binding domain interaction with F-actin
a, Anti-GFP immunoblot on lysates from NIH-3T3 cells transiently transfected with GFP or FLNaABD-GFP ± ABS2ʹ mutations. Vinculin was used as a loading control. WT, wild type. b, Anti-GFP immunoblot on lysates from NIH-3T3 cells transiently transfected with GFP or FLNaABD-GFP ± ABS2 mutations. Vertical separations between panels indicate the exclusion of non-relevant lane samples from the same gel. Vinculin was used as a loading control.
