Supplementary Figure 2: SdFLIC example images and evaluation for Syx*192/SNAP-25/Syb(1-96) in different lipid environments.

a–l, Example sdFLIC images with a yellow grid of 12 × 12 oxides that were evaluated, extracted intensity data from one set of 16 oxides and FLIC fit curve, and example histogram of fit results obtained from one sample under one condition for Syx*192/SNAP-25/Syb(1-96). Images in a–f were taken before and after the addition of Ca²⁺/C2AB in different lipid headgroup environments, as described in Fig. 1d and indicated in each panel. Images in g–l were taken in different lipid environments, as described in Fig. 2a and indicated in each panel. Images were rotated and scaled for printing and show an area of 201 × 201 μm².