Supplementary Figure 3: Delayed macroH2A2 deposition at a subset of steady-state peaks is associated with heterochromatic features (related to Fig. 2).
From: Transcription-associated histone pruning demarcates macroH2A chromatin domains
(a) Spearman correlation heatmap of nChIP-seq profiles genome-wide in DFs10 and iDFs. Black box highlights macroH2A nChIPs. (b) Heatmap of macroH2A relative enrichment at all macroH2A peaks in WT DFs. (c) Separation of steady-state peaks into three categories based on the time when they are first detected after dox induction by nChIP-seq. (d) Box plots of H3K27me3 (DF)10 and H3K9me3 (MEF)25 ChIP-seq occupancy level at early (E), intermediate (I) and late (L) steady-state peak regions of macroH2A2. Boxes span the lower to upper quartile; median is indicated with a black line; whiskers show locations of the minimum and maximum; P values are calculated using a two-tailed t test, **** P < 2.2 × 10−16.
