Supplementary Figure 5: MCDs are flanked by actively transcribed chromatin across differentiated cell types (related to Fig. 4). | Nature Structural & Molecular Biology

Supplementary Figure 5: MCDs are flanked by actively transcribed chromatin across differentiated cell types (related to Fig. 4).

From: Transcription-associated histone pruning demarcates macroH2A chromatin domains

Supplementary Figure 5

(a) Size distribution of all MCDs in iDFs. (b) Observed over expected random distribution of active and inactive genes falling within MCDs. The dashed line represents the expected value of a random distribution. Error bars represent s.d. P values are calculated using Monte Carlo testing, *** P < 0.001. (c) Metagene plots of Pol II S2P (MEF)27, total Pol II (MEF)29, H3K27me3 (DF)10, H3K27ac (DF)10, and H2A.Z (DF) occupancy at iDF MCDs. (d-e) Metagene plots of fetal liver and C2C12 myoblast MCDs18,28, occupancy of Pol II29,30 and indicated histone modifications29 of respective cell types at corresponding MCDs.

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