Supplementary Figure 6: CDS-targeted miRNA does not alter overall polysome profiles.
From: A novel class of microRNA-recognition elements that function only within open reading frames
a, Luciferase activities (upper panel) and relative mRNA levels determined by real-time RT-PCR (lower panel) in three stable HeLa cell lines expressing the luciferase reporter carrying the miR-20a MRE in different locations (note that, as shown in Fig. 2a, the native MRE is non-functional in 3’UTR, which served as control). b,c,d, Polysome profiling by sucrose gradients on the three stable HeLa cell lines. OD260 traces are shown below individual constructs and the distribution of mRNAs from individual reporters and the endogenous Actin gene is shown at bottom. The mRNA levels were determined by real-time RT-PCR on individual fractions and expressed as percentage of the sum. The data shown in a,b,c,d were based on 3 independent experiments. *p<0.05; **p<0.01; ***p<0.001 (Student’s t-test).
