Supplementary Figure 7: Binding of PTH(1–34) to wild-type and mutant variants of PTH1R. | Nature Structural & Molecular Biology

Supplementary Figure 7: Binding of PTH(1–34) to wild-type and mutant variants of PTH1R.

From: High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist

Supplementary Figure 7: Binding of PTH(1–34) to wild-type and mutant variants of PTH1R.

Binding of PTH-HL647 to HEK293T cells expressing wild-type and mutated PTH1R variants in the presence of different concentrations of unlabeled wt PTH(1–34) as a competitor. Data are shown as mean values ± s.e.m. from 4–12 independent experiments performed in duplicate. The corresponding IC50 values and numbers of independent experiments for wt and mutant PTH1Rs are listed in Supplementary Table 2.

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