Supplementary Figure 1: Protein purification and workflow of cryo-EM image processing.
From: Cryo-EM structure of the yeast respiratory supercomplex

a, BN PAGE of total mitochondria (T) and elution (E) after purification. b, A representative cryo-EM micrograph from dataset 3. The scale bar is set to 390 Å. c, Representative 2D classes show multiple orientations and classes. The box size is 370 pixels with a pixel size of 1.06 Å. d, Workflow of cryo-EM image processing.