Supplementary Figure 4: Cryo-EM structure analysis of the ISW1–nucleosome complex in the ADP-BeFx-bound state fixed with glutaraldehyde.
From: Structures of the ISWI–nucleosome complex reveal a conserved mechanism of chromatin remodeling
(a) A representative Cryo-EM micrograph. (b) FFTs of image in (a) with the Thon rings extending to ~3.5 Å. (c) 2D class averages of characteristic projection views of Cryo-EM particles. (d) Flowchart of Cryo-EM data processing for the ISW1-152NCP-ADP-BeFx-crosslinked dataset. (e) Angular distributions of cryo-EM particles in the final round of refinements of the ISW1-NCP-complex sample. (f) Angular distributions of cryo-EM particles in the final round of refinements of the ISW1-NCP-SHL-2-complex sample. (g) Angular distributions of cryo-EM particles in the final round of refinements of the ISW1-NCP-SHL2-complex sample. (h) Resolution estimation of the EM maps. Gold standard Fourier shell correlation (FSC) curves, showing the overall nominal resolutions of 3.25 Å, 3.37 Å, 3.61 Å, 3.77 Å for the NCP masked region of the ISW1-NCP-complex, ISW1-NCP-complex, ISW1-NCP-SHL-2-complex and ISW1-NCP-SHL2-complex, respectively.
