Supplementary Figure 7: Structural changes of ISW1 after activation.
From: Structures of the ISWI–nucleosome complex reveal a conserved mechanism of chromatin remodeling
(a) Superimposition of the structure of ISW1 (color coded) in the ADP-BeFx- nucleosome-bound state and that of MtISWI in the auto-inhibited state (grey, PDB code 5JXR) (Nature 540, 466–469, 2016). Only the region around the catalytic pocket is shown. The alignment was performed on core1 of the enzymes. (b) Superimpositions of the structure of core1 of ISWI in the activated state (color coded) and the inhibited state (grey). Dashed line, the disordered region of AutoN in the activated state. (c) Interactions with the histones H4 and H3 by ISW1 in the context of the nucleosome. (d) Comparison of the H4-binding modes in the contact of the nucleosome and free H4 peptide. MtISWI (PDB code 5JXT), grey; H4 peptide, magenta. Structural alignment was performed on core2 of the enzymes.
