Supplementary Figure 1: PICH cooperates with TRR to induce DNA positive supercoiling.
From: PICH and TOP3A cooperate to induce positive DNA supercoiling
(a–c) Representative 1D agarose gel electrophoresis showing the influence of increasing concentrations of PICH on the topoisomerase activity catalyzed by hTop1 (a), hTop2a (b), and TRR (c) using a negatively supercoiled plasmid as a substrate. Agarose gel electrophoresis was either performed in the absence (neutral; a, b) or in the presence of chloroquine (c) in order to reveal variations of topology within the negatively supercoiled topoisomers. For each experiment, relaxed (rel), negatively (-) moderately positively (rel+), and positively (+) supercoiled plasmids were used as markers. (d) Representative 2D agarose gel electrophoresis of the reaction products shown in (c). For each panel, representative images of at least 3 independent experiments are presented. Each independent experiment lead to similar results.
