Supplementary Figure 7: Xrn1-mEGFP validation.

(a) Northern Blot for RPL28 mRNA in yeast cultures expressing either wild type Xrn1 (WT), Xrn1-mEGFP or no Xrn1 expression (xrn1Δ). Pre-mRNA of RPL28 is efficiently processed by Xrn1-mEGFP comparable to wild type. (b) The histogram represents the mean ratio (for three biological replicates) of RPL28 pre-mRNA over total (pre-mRNA + mRNA) for the wild-type reference strain BY4742, the Xrn1-mEGFP mutant and the xrn1∆ mutant, quantified from a northern blot analogous to the one presented in Supplementary Fig. 1c. Error bars represent the standard deviations. (c) Fluorescence microscopy of yeast wild type cells (WT) compared with Xrn1-mEGFP strain.