Supplementary Figure 2: 3D classification of the 80S–Xrn1 cryo-EM dataset. | Nature Structural & Molecular Biology

Supplementary Figure 2: 3D classification of the 80S–Xrn1 cryo-EM dataset.

From: Structure of the 80S ribosome–Xrn1 nuclease complex

Supplementary Figure 2: 3D classification of the 80S–Xrn1 cryo-EM dataset.

1,633,111 particles were initially separated into eight classes partly representing different translational states of the ribosome. 60 % of the particles containing tRNAs in the P- and E sites were joined and separated into two classes with and without Xrn1 (highlighted in blue throughout the figure). The class containing Xrn1 was refined to an overall resolution of 3.1 Å after post-processing followed by a two-step focused classification using a soft mask for the Xrn1 density. The final Xrn1-containing class was focused-refined to a resolution of 3.6 Å.

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