Supplementary Figure 2: Fz–Fz dimer formation.
a, The 2:2 symmetric dimer found in the hWnt3–mFz8 CRD crystal. The upper panel shows three independent hWnt3/mFz8 CRD units (chains AB, CD, and EF) shown with their respective packing mates (chains A’B’, C’D’, and E’F’). The bottom panel shows the crystal structure of apo-form of Fz8 CRD (chains A and B, PDB ID: 1IJY) drawn with their packing mate (chains A’ and B’), PAM-bound Fz5 CRD (chain B, PDB ID: 5URY) drawn with its packing mate (chain B’), as well as a pseudosymmetric dimer of Fz7 CRD bound by a C24 fatty acid (chains A and B, PDB ID: 5URV). The 2-fold symmetry axis is denoted by dotted lines. Lipid molecules are drawn as sphere models. b, Lipophilic cavity at the Fz-Fz interface. The volume of the cavity formed at the Fz-Fz symmetric dimer interface in the 7 dimeric structures in (a) are calculated by using the ChannelFinder program in the 3V server (http://3vee.molmovdb.org/) with the outer and inner probe radius set to 4.0 and 1.4 Å, respectively. The volume map files (.mrc format) produced by the server are opened in UCSF Chimera 1.7.0 and drawn with Fz CRD structures as in (a). The volume of the PAM chain moiety taken from chain B was calculated by the VolumeCalculation program in the same server, resulting in a value of 298 Å3.
