Supplementary Figure 3: Gel filtration profiles of various protein preparations.

a,b, Mouse Wnt3a in complex with mFz8 CRD prepared exactly as hWnt3–mFz8 CRD complex used in the crystallization experiments was loaded to a Superdex 200 gel filtration column at either 200 µM (a, 11 mg/ml) or 9 µM (b, 0.5 mg/ml) and eluted with HBS. The estimated molecular sizes for each peak were calculated from the elution positions of standard marker proteins. c,d, Purity and the monodispersity of the LRP6(3–4) (c) and EGFRec (d) preparations used in the SPR experiments were confirmed by gel filtration on a Superdex 200 column (upper panels) and the SDS-PAGE analysis (lower panels).