Supplementary Fig. 5: Only NSUN2 regulates the mRNA m5C in HeLa cells.
From: Genome-wide identification of mRNA 5-methylcytosine in mammals
(a) The expression levels of NSUN methyltransferase family and DNMT2 in HeLa cells. Data was obtained from the Human Protein Atlas (https://www.proteinatlas.org). (b) Comparison of the expression levels of NSUN2, NSUN3, NSUN4 and NSUN6 in control and knockdown samples. mRNA expression was measured using RT-PCR. Three technical replicates were performed. P-values were calculated using two-sided Student’s t-test: (∗∗) P < 0.01; (∗∗∗) P < 0.001. Error bars denote standard deviation. (c) Western blot analysis of NSUN2, NSUN4 and NSUN6 upon siRNA knockdown. NSUN3 data was not shown because the NSUN3 antibody does not work. (d) Boxplot showing m5C level between control and knockdown HeLa cells. m5C sites identified in the control sample were used for analysis. Box boundaries represent 25th and 75th percentiles; center line represents the median; whiskers indicate ± 1.5 × IQR.
