Supplementary Fig. 4: Structural superposition of DRPB in complex with Fz CRDs.
From: Receptor subtype discrimination using extensive shape complementary designed interfaces
a, Superposition of the overall structures of the two complexes. The RMSD between the two complexes is 0.6 Å. Fz7 is colored in pink. Fz8 is colored in blue. DRPB_Fz7/8 is colored in cyan. DRPB_Fz8 is colored in orange. b, Zoomed view of Met9Arg mutation from DRPB_Fz8 to DRPB_Fz7/8. The Met9Arg mutation leads to hydrogen bond and salt bridge formation between Arg9 with Fz7 Asn75 and Asp78, as shown by grey dashed lines. c, Superposition of the overall structures of the DRPB_Fz7/8-Fz7 and DRPB_Fz7-Fz7. Fz7 is colored in light orange. DRPB_Fz7/8 is colored in light cyan. DRPB_Fz7 is colored in light blue. In the zoomed view, the carboxylate group of Asp112 forms a hydrogen bond and salt bridge with Lys46 of Fz7 CRD, enhancing the affinity.
