Supplementary Fig. 8: Cryo-EM densities of PF846-stalled nascent chains. | Nature Structural & Molecular Biology

Supplementary Fig. 8: Cryo-EM densities of PF846-stalled nascent chains.

From: Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule

Supplementary Fig. 8: Cryo-EM densities of PF846-stalled nascent chains.

Local resolution estimation for the PF846 stalled nascent chains from a, CDH1-RNC, b, PCSK9-RNC and c, USO1-RNC in the rotated state with A/P NC-tRNA, shown in the same orientation. The asterisks indicate the location of the peptidyl transferase center. d, e, Density map in the region where the (d) CDH1 (purple) and (e) PCSK9 (blue) nascent chains interact with the ribosome exit tunnel. Map density is shown in mesh, ribosomal proteins and rRNA nucleotides that have interaction with NC are shown in stick form (uL4 in green, uL22 in orange, eL39 in pink and 28S rRNA in cyan). The atomic models of the nascent chains are also shown in stick representation.

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