Supplementary Fig. 3: Cryo-EM data processing of PF846 stalled PCSK9-RNCs. | Nature Structural & Molecular Biology

Supplementary Fig. 3: Cryo-EM data processing of PF846 stalled PCSK9-RNCs.

From: Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule

Supplementary Fig. 3: Cryo-EM data processing of PF846 stalled PCSK9-RNCs.

a, Data processing steps for stalled PCSK9-RNCs, as described in Supplementary Fig. 2. Particle numbers at various steps of the reconstruction process are indicated. b, Final FSC curves of PCSK9-RNCs, with the “gold standard” value of 0.143 used to define the resolution indicated. Particle numbers used for the final reconstructions are indicated. c, Model to map correlations with resolution at FSC value of 0.5. d, Data processing steps for sample prepared with a short incubation time, which generated a higher ratio of non-rotated to rotated states of the ribosome. e, Final FSC curves of RNCs, with the “gold standard” value of 0.143 used to define the resolution as indicated.

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