Supplementary Figure 4: Paramagnetic relaxation enhancement within the condensed phase.
From: Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain
A) 1H transverse relaxation rates of condensed FUS LC containing ~1% Ac-MTSL labeled A16C, S86C, and S142C. Labeling at position S86C was repeated (S86C #2) and shows a similar relaxation rate as the original experiment (S86C #1). Data are plotted as mean ± best-fit parameter confidence interval equal to 1 s.d. in one data set, with two data sets plotted for S86C. B) 1H transverse relaxation rates of condensed FUS LC containing ~1% MTSL labeled A16C, S86C, and S142C. C-E) Signal intensity ratios of the condensed FUS LC diamagnetic MTSL and paramagnetic MTSL. The global decrease in signal intensity indicates labeling with paramagnetic MTSL. Data are plotted as peak height ratio mean ± propagated uncertainty equal to r.m.s.d. of baseline noise in the representative data set of two experiments.
