Supplementary Figure 7: Substitution of tyrosines with phenylalanine in FUS LC leads to aggregation.
From: Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain
A) DIC microscopy of a FUS LC variant in which all 24 tyrosines are mutated to phenylalanine (FUS LC YF). This variant forms aggregates instead of spherical droplets. Phase separation as monitored by turbidity of MBP-FUS FL WT (B) or YF (C). The increased turbidity and light scattering and long incubation time in the YF variant indicates the presence of stable aggregates. Data are plotted as mean ± s.d. of n=3 technical replicates.
