Supplementary Figure 1: Electrophysiological properties of spHCN channels with incorporated Anap.
From: The HCN channel voltage sensor undergoes a large downward motion during hyperpolarization
a, Left: representative current traces of wild-type spHCN channels in the absence or the presence of 1 mM cAMP. In the absence of cyclic nucleotide, the spHCN channel rapidly inactivates with hyperpolarization. Right: representative current traces from wild-type spHCN channels elicited by a series of hyperpolarizing voltage pulses from 0 mV to -110 mV with 10 mV steps, in the presence of 1 mM cAMP. b, Representative current traces from spHCN channels with L-Anap incorporated at different sites elicited by a series of hyperpolarizing voltage pulses from 0 mV to -120 mV, in the presence of 1 mM cAMP.
