Supplementary Figure 3: Emission spectra for L-Anap at each position in spHCN.
From: The HCN channel voltage sensor undergoes a large downward motion during hyperpolarization
a-e, Emission spectra of L-Anap measured with a spectrograph for the indicated positions in spHCN at 0 mV and -100 mV. These spectra were fit with emission spectra for free L-Anap in solution, measured with a fluorometer shifted so that the peak positions are the same (black traces). The small peak at about 530 nm most likely corresponds to direct excitation of YFP. Also shown is the absorption spectra of Cu2+-TETAC (blue). f, Summary of the peak wavelength of the Anap emission for the various Anap sites in spHCN (n = 3 - 4, *p < 0.05). Error bars are s.e.m.
