Supplementary Figure 3: Crystal structure of the MIR domain of Pmt2.
From: Structure of the eukaryotic protein O-mannosyltransferase Pmt1−Pmt2 complex
(A) Gel filtration of the MIR domain of Pmt2. The inset shows the SDS-PAGE gel of the five fractions collected around the filtration peak (shown by the horizontal arrow). (B) 3D electron density map and atomic model of a selected region. (C) Overall structure of the MIR domain of Pmt2 shown in a top, bottom, and side views. The structure contains three repeats of the MIR motif, each of which has four β-strands and one short α-helix. The numbers refer to the β-strand number in the β-trefoil fold. All residues in the Pmt2 MIR domain (amino acids 337-532) are resolved (i.e., no residues are missing in the crystal structure).
