Supplementary Figure 5: DNA fiber dual-pulse experiments in bam mutant testis. | Nature Structural & Molecular Biology

Supplementary Figure 5: DNA fiber dual-pulse experiments in bam mutant testis.

From: Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement

Supplementary Figure 5: DNA fiber dual-pulse experiments in bam mutant testis.The alt text for this image may have been generated using AI.

(a) A cartoon showing experimental protocol. (b) Predicted unidirectional fork progression result. (c) Unidirectional fork progression pattern from germline-derived chromatin fiber. Multiple replicons show alternation between early label (EdU in magenta) and late label (BrdU in cyan) along one chromatin fiber toward the same direction. DNA label (DAPI) shows continuity between replicons. (d) Cartoon representation of wild-type testes versus bam mutant testes. (e) Replication patterns in bam mutant testis. No category of fork movement (unidirectional, asymmetric bidirectional or bidirectional) shows statistically significant differences from wild-type testes. Chi-squared test: WT Testis vs. bam mutant testis. Unidirectional frequency: The chi-square statistic is 0.1169. The p-value is .732432 Asymmetric bidirectional frequency: The chi-square statistic is 0.0821. The p-value is .774529. Symmetric bidirectional frequency: The chi-square statistic is 0.3903. The p-value is .532159.

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