Supplementary Figure 4: Local resolution and validation of the cryo-EM maps.
From: The molecular mechanism of cotranslational membrane protein recognition and targeting by SecA
a, Local resolution of Map1 obtained from the 3D refinement (left), and close-up of the contact points and the resolved density for the RodZ TMD at a similar resolution as the overall resolution (right). b, Comparison of the local resolution plot for the EM density corresponding to SecA using the global refinement approach (left) and the focused refinement approach (right). Corresponding color keys are shown on the right side of each map. c, Fourier Shell Correlation (FSC) plots for Map1 (3.1 Å) and Map2 (5.7 Å) using the gold-standard FSC criteria cutoff (FSC = 0.143) using independent two half maps as implemented in RELION3 and cryoSPARC. Map1 versus obtained model plot shown in red and depicts a similar resolution 3.3 Å using cutoff (FSC = 0.5) as that of the cryo-EM Map1.
