Extended Data Fig. 7: PRMT5 depletion affects the binding of SRSF1 to mRNA and proteins.
From: PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia
a, Western blotting for SRSF1 and PRMT5 in the input and immunoprecipitation samples (either SRSF1 or IgG). Bar chart shows quantification of protein levels. b, RNA yield after RNA-immunoprecipitation and purification in three biological replicates of each sample. c, Heatmap of the methylated peptides identified for SRSF1 in the negative control and PRMT5 KD SRSF1 IP-MS samples. “aa” stands for amino acid. Each IP was performed in three biological replicates. The uncropped western blots are available in the Source Data.
