Supplementary Figure 1: Details of crystal structures and changes in NMR intensities in the MYC:TBP:TAF1 complex.
From: Multiple direct interactions of TBP with the MYC oncoprotein
a, Cartoon rendering of PDB_ID 6E24, highlighting TBP (green), TAF1 (cyan), and MYC (yellow). Key elements of secondary structure of TBP are shown; helices 2 and 2’ (H2, H2’) and N- and C-lobes of TBP are labelled to designate orientation. b, Cartoon rendering of PDB_ID 6E16 in the same orientation as in a. c, 2Fo-Fc electron density map (blue) of MYC peptide (in yellow backbone) in T3M3T contoured at 1.2 s with key residues labelled. d, 2Fo-Fc electron density map (blue) of MYC peptide (in orange backbone) in T1M3T contoured at 1.2 s with key residues labelled. e, Overlay of the PDB_ID 6E24 and 6E16 structures, MYC from 6E24 in yellow and MYC from 6E16 in orange. f, Overlay of the 6E24 structure with the TBP-DNA structure (1YTB), superimposing on TBP. g, NMR intensity changes (I/I0) on MYC binding to TBP:TAF1, mapped onto the TBP:TAF1 (4B0A) structure, rendered as a cartoon. Residues of TBP and TAF1 that broaden beyond detection are coloured in green and cyan, respectively. h, same as g, but rotated 90º.
