Extended Data 3: Structural details and size exclusion chromatography of Mtb-ileS with tRNAIle-cP. | Nature Structural & Molecular Biology

Extended Data 3: Structural details and size exclusion chromatography of Mtb-ileS with tRNAIle-cP.

From: Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators

Extended Data 3

a, Tertiary structure model of Mtb-ileS in complex with tRNAIle-cP. b, Detail of AntiS interaction with tRNAIle-cP NCCA sequence. Hydrogen bonds represented by black dashes. Asterisk indicates residues mutated from wildtype sequence. c, Size exclusion chromatograms of Mtb-ileS folded with tRNAIle-cP (blue) and with tRNAIle-OH (orange). d, Urea denaturing PAGE gel of peak fractions from T-box + tRNAIle-cP peak.

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