Extended Data 4: Mechanistic diagram of atypical T-box translational regulation.
From: Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators
a, tRNA recruitment and decoding. b, Pseudoknot formation positions AntiS to interact with tRNA NCCA sequence. c, Transient intermediate where aminoacylation sensing module interacts with uncharged (top) and charged (bottom) tRNA. d, Favorable interactions between uncharged tRNA and linker locks T-box into ON conformation. Exposed Shine–Dalgarno (SDS) allows translation initiation. e, Steric clashing between charged tRNA and linker unravels the aminoacylation sensing module leading to alternative base pairing. Sequestrator formation prevents ribosome access to SDS and prevents translation.
