Extended Data Fig. 7: Conserved residues in all RAF isoforms stabilize ACP induced inactive state, which is disrupted by activating mutations like V600E in BRAF.
From: Negative regulation of RAF kinase activity by ATP is overcome by 14-3-3-induced dimerization
a, Alignment of ARAF, BRAF and CRAF across species shows conservation of key residues involved in the hydrophobic packing interactions displayed in Fig. 3c. b, BRAFKD-V600E:MEK1 complex alone (top), in the presence of ACP (middle) and in the presence of GDC-0879 (bottom) from SEC experiments in main Fig. 3c were analyzed by SDS-PAGE. The positions of MEK1 and BRAFV600E are labeled to the right of each gel.
