Extended Data Fig. 9: Functional interacting residues between MlaB and MlaF.
From: Structural insights into outer membrane asymmetry maintenance in Gram-negative bacteria by MlaFEDB
a, Residues from the C-terminal tail of MlaF show interactions with the opposite MlaF and MlaB. b, SDS-PAGE analysis of purified MlaFEDB wildtype or mutant containing single mutation of the signature motif residues mutants E144A, S146S, R151A of MlaF, and C-terminal residues Y256D and H262D of MlaF. c, The relative ATPase activity of MlaFEDB mutants in detergent and in liposomes. d, FRET scan of PL transport assay using wildtype MlaFEDB or mutant IM proteoliposomes, wildtype OM proteoliposome and MlaC for retrograde direction. e, Cellular sensitivity to chlorpromazine by the mutant MlaF(Y256D)EDB or MlaF(H262D)EDB, showing no cellular effect. f, Leaky expression of pTRC99a_MlaFEDCB carrying mutations on C-terminal residues. g, SDS-PAGE analysis of purified MlaFEDB and mutant with MlaF being truncated at the C-terminal tail MlaF(ΔI247-S269)EDB. h, Leaky expression of pTRC99a_MlaFEDCB carrying mutations on signature motif residues. Data in b, d-h are representative results from n = 3 independent experiments. Data in c represents mean ± s.d. (n = 3 independent experiments). Uncropped images are available online. Source data for panels c and d are available in Supplementary Data Set 1.
