Extended Data Fig. 1: Sample preparation and cryo-EM analysis of human TLR3-UNC93B1 complex.
From: Cryo-EM structures of Toll-like receptors in complex with UNC93B1
a Representative size-exclusion chromatography profile of human TLR3-UNC93B1 complex (left) and SDS-PAGE analysis of the purified sample stained with Coomassie blue (right). Pooled fractions are shown with the bar. Absorbances at 280 nm and 260 nm are shown with solid and dashed lines, respectively. An uncropped image is available as Supplementary Data. b Data processing workflow of cryo-EM analysis of human TLR3-UNC93B1 complex. Representative motion-corrected micrograph, 2D class averages, and 3D class-averages, gold-standard FSC curve of the final 3D reconstruction (resolution cutoff at FSC = 0.143), and the final 3D-map colored according to the local resolution are shown. The 2D class averages were calculated using the refined particles that was used for the final reconstruction. The 3D classes selected for following analysis are indicated with red boxes.
