Extended Data Fig. 1: Purification of FANCI, FANCD2 and D2–I, and cryo-EM of D2–I, ubD2–I and D2 dimer.
From: FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
a, Coomassie gel showing purified His-tagged FANCI, StrepII-tagged FANCD2 and D2–I after gel filtration. b–e, Cryo-EM of D2–I, ubD2–I and D2. b, Representative micrographs. Selected individual particles are marked with green circles. Scale bars, 25 nm. c, Fourier shell correlation (FSC) curves for gold-standard refinements. d, Angular distribution density plots of particles used in 3D reconstructions calculated using cryoEF63. Every point is a particle orientation, and the color scale represents the normalized density of views around this point. The color scale runs from 0 (low, blue) to 0.00026 (high, red). All complexes had a preferred orientation. Note that C2 symmetry was applied for FANCD2. e, Local resolution estimates calculated using ResMap64. Uncropped image for a is available in Supplementary Fig. 1.
