Extended Data Fig. 4: FANCD2 and FANCI oligomerization state and DNA binding activity.
From: FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
a, Size-exclusion chromatogram as shown in Fig. 5a (top). Peak fractions were analyzed via SDS−PAGE (bottom). A single asterisk (*) indicates the migration position for monomers. A double asterisk (**) indicates the migration position for dimers. These results are representative of experiments performed three times. b, DNA binding of FANCI, FANCD2, D2–I and FANCI mixed with FANCD2 was analyzed in EMSAs performed with 20 nM 39-bp dsDNA and 0–140 nM protein. Gels shown are representative gels of experiments performed independently three times. The FANCD2 and D2–I gels are same as in Fig 5b. c, Quantification of mean intensities of free DNA from b. Error bars represent the standard deviation. Individual data points (n = 3 independent experiments) are shown. The means are connected by lines for clarity. Uncropped images for a,b are available in Supplementary Fig. 1. Data for the plot in c are available as Source data.
