Extended Data Fig. 3: Aggregation kinetics of ΔC1 and P1P2-GS.
From: A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function
a, Schematic of WT, ΔC1, ΔΔ and P1P2-GS αSyn variants including the amino acid sequence of the deleted C1 region and substituted P1-P2 region. b−g, ThT assays at pH 4.5 or pH 7.5 of WT αSyn (b,c), ΔC1 (Δ14−20) (d,e), or P1P2-GS (f,g). Dark and light colors shows assays in high- (200 mM added NaCl) and low-salt (20 mM added NaCl) conditions, respectively. h,i, Negative-stain TEM images of representative samples at the endpoint (100 h) of the incubation of ΔC1 (h) or P1P2-GS (i), with same color scheme as in c−f. Scale bars, 200 nm. The fibril yield under each condition, determined by SDS−PAGE after centrifugation (Methods) is shown in Supplementary Table 1. Data for graphs in b−g are available as Source data.
