Extended Data Fig. 6: Primer extension by Pol δ and Pol ε.
From: Rad53 limits CMG helicase uncoupling from DNA synthesis at replication forks
a, Reaction scheme: Singly primed single-stranded M13mp18 DNA was pre-incubated with RPA, RFC/PCNA, three nucleotides, and Pol δ to initiate primer extension; Pol ε was subsequently added along with the remaining fourth nucleotide and incubation continued for 3 minutes. b, Denaturing agarose gel analysis of primer extension products obtained according to reaction scheme in a, but with either Pol δ or Pol ε omitted from the reaction. Uncropped gel images are available as source data.
